FD5180, a novel protein kinase affinity probe, and the effect of bead loading on protein kinase identification

Deane, Fiona M.; Lin, Andrew J. S.; Hains, Peter G.; Pilgrim, Sarah L.; Robinson, Phillip J.; McCluskey, Adam

Title: FD5180, a novel protein kinase affinity probe, and the effect of bead loading on protein kinase identification
Creator: Deane, Fiona M.; Lin, Andrew J. S.; Hains, Peter G.; Pilgrim, Sarah L.; Robinson, Phillip J.; McCluskey, Adam
Relation: ACS Omega Vol. 2, Issue 7, p. 3828-3838
Publisher Link: http://dx.doi.org/10.1021/acsomega.7b00020
Publisher: American Chemical Society
Resource Type: journal article
Date: 2017
Description: The effects of compound loading on the identification of protein kinases (PKs) was examined using two previously reported sepharose-supported PK inhibitors (PKIs): bisindolylmaleimide X (S1) and CZC8004 (S2). Compound loadings of 0.1, 0.5, 2.5, 5, 10, 25, and 50% content and an ethanolamine-blocked control bead (no compound) were investigated. A 50% bead loading gave the highest level of PK identification for both S1 and S2, extracting 34 and 55 PKs, respectively, from a single cell lysate. Control beads allowed overall identification of 23 PKs, which we term the kinase beadome, whereas sepharose-supported sunitinib (S7; 50% loading) identified 20, 11 of which were common to the control beads. The reliability of bead pull-downs was examined in duplicate experiments using two independently synthesized batches each of S1 and S2. Bead S1 showed high similarity in the absolute numbers of PKs identified across two experiments, at 40 and 35 PKs, of which 26 were common across the two batches of beads, with 14 and 9 unique PKs identified in each experiment. The S2 beads extracted 61 and 64 PKs with 55 PKs common across the two bead batches examined. We also report on the development and use of a novel promiscuous PKI analogue, 2-[(5-chloro-2{[4-(piperazin-1-yl)phenyl]amino}pyrimidin-4-yl)amino]-N-methylbenzene-sulfonamide (S15), which extracted 12 additional unique PKs over the two parent compounds from which it was designed, the combination of which identifies 160 unique PKs. S15 was based on the common pyrimidine core scaffold of S9 and S10. Thus, S15 expands the utility of kinobeads by broadening the kinome coverage for bead-based pull-down. Combining the data for all beads across 90 and 180 min liquid chromatography–mass spectrometry (LC–MS)/MS analysis identified a total of 160 unique PKs.
Subject: cell biology; molecular biology; liquid chromatography; FD5180; protein kinases; protein kinases identification; bead loading; protein kinase affinity probe
Identifier: http://hdl.handle.net/1959.13/1396507
Identifier: uon:34053
Identifier: ISSN:2470-1343
Language: eng
Full Text
Reviewed

Hits: 5232
Visitors: 5792
Downloads: 347

		Thumbnail	File	Description	Size	Format
View Details Download			ATTACHMENT02	Publisher version (open access)	2 MB	Adobe Acrobat PDF	View Details Download